pAd/CMV/V5-DEST vector (Cat. No.: V011866)
Basic Information
- Name:
- pAd/CMV/V5-DEST
- Antibiotic Resistance:
- Ampicillin
- Length:
- 36686 bp
- Type:
- Gateway Cloning Vectors
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
pAd/CMV/V5-DEST vector (Cat. No.: V011866) Sequence
LOCUS V011866 36686 bp DNA circular UNA 01-JAN-1980
DEFINITION Exported.
ACCESSION V011866
VERSION V011866
KEYWORDS pAd CMV V5-DEST
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
.
REFERENCE 1 (bases 1 to 36686)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 36686)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..36686
/mol_type="genomic DNA"
/organism="synthetic DNA construct"
repeat_region 1..103
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
misc_signal 191..341
/label="Ad5 Psi"
/note="packaging signal for adenovirus serotype 5"
enhancer 731..1110
/label="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 1111..1314
/label="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 1359..1377
/label="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1407..1531
/label="attR1"
/note="recombination site for the Gateway(R) LR reaction"
CDS complement(1963..2265)
/label="ccdB"
/note="CcdB, a bacterial toxin that poisons DNA gyrase"
CDS complement(2610..3266)
/label="CmR"
/note="chloramphenicol acetyltransferase"
promoter complement(3267..3369)
/label="cat promoter"
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
protein_bind complement(3547..3671)
/label="attR2"
/note="recombination site for the Gateway(R) LR reaction"
CDS 3697..3738
/label="V5 tag"
/note="epitope tag from simian virus 5"
polyA_signal 3822..3870
/label="HSV TK poly(A) signal"
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
CDS 4152..4571
/gene="IX"
/label="Hexon-interlacing protein"
/note="Hexon-interlacing protein from Human adenovirus C
serotype 5. Accession#: P03281"
CDS complement(4637..5971)
/gene="IVa2"
/label="Packaging protein 1"
/note="Packaging protein 1 from Human adenovirus C serotype
5. Accession#: P03271"
CDS complement(5743..9324)
/gene="POL"
/label="DNA polymerase"
/note="DNA polymerase from Human adenovirus C serotype 2.
Accession#: P03261"
CDS complement(9129..11132)
/gene="PTP"
/label="Preterminal protein"
/note="Preterminal protein from Human adenovirus C serotype
5. Accession#: P04499"
CDS 11593..12837
/gene="L1"
/label="Packaging protein 3"
/note="Packaging protein 3 from Human adenovirus C serotype
5. Accession#: P04496"
CDS 12861..14615
/note="Pre-hexon-linking protein IIIa from Human adenovirus
C serotype 5. Accession#: P12537"
/label="Pre-hexon-linking protein IIIa"
CDS 14699..16411
/gene="L2"
/label="Penton protein"
/note="Penton protein from Human adenovirus C serotype 5.
Accession#: P12538"
CDS 16421..17014
/gene="L2"
/label="Pre-histone-like nucleoprotein"
/note="Pre-histone-like nucleoprotein from Human adenovirus
C serotype 2. Accession#: P68950"
CDS 17087..18190
/gene="L2"
/label="Core-capsid bridging protein"
/note="Core-capsid bridging protein from Human adenovirus C
serotype 5. Accession#: P24938"
CDS 18221..18460
/note="Pre-core protein X from Human adenovirus C serotype
5. Accession#: Q2KS10"
/label="Pre-core protein X"
CDS 18546..19295
/gene="L3"
/label="Pre-protein VI"
/note="Pre-protein VI from Human adenovirus C serotype 5.
Accession#: P24937"
CDS 19385..22240
/gene="L3"
/label="Hexon protein"
/note="Hexon protein from Human adenovirus C serotype 5.
Accession#: P04133"
CDS 22276..22887
/gene="L3"
/label="Protease"
/note="Protease from Human adenovirus C serotype 5.
Accession#: P03253"
CDS complement(22989..24575)
/gene="DBP"
/label="DNA-binding protein"
/note="DNA-binding protein from Human adenovirus C serotype
5. Accession#: P03265"
CDS 24604..27024
/gene="L4"
/label="Shutoff protein"
/note="Shutoff protein from Human adenovirus C serotype 5.
Accession#: P24933"
CDS 27717..28397
/gene="L4"
/label="Pre-hexon-linking protein VIII"
/note="Pre-hexon-linking protein VIII from Human adenovirus
C serotype 5. Accession#: P24936"
CDS 29707..31449
/note="Fiber protein from Human adenovirus C serotype 5.
Accession#: P11818"
/label="Fiber protein"
CDS complement(31861..32742)
/note="Early 4 ORF6 protein from Human adenovirus C
serotype 2. Accession#: P03239"
/label="Early 4 ORF6 protein"
CDS complement(33021..33368)
/note="Early 4 ORF3 protein from Human adenovirus C
serotype 2. Accession#: P03241"
/label="Early 4 ORF3 protein"
CDS complement(33809..34192)
/note="Early 4 ORF1 protein from Human adenovirus C
serotype 2. Accession#: P03242"
/label="Early 4 ORF1 protein"
repeat_region 34502..34604
/label="ITR"
/note="inverted terminal repeat of human adenovirus
serotype 5"
rep_origin complement(34828..35416)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(35571..36428)
/label="AmpR"
/note="beta-lactamase"
promoter complement(36429..36533)
/label="AmpR promoter"
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