pAd/PL-DEST vector (V011865)

LOCUS       V011865                34864 bp    DNA     circular UNA 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011865
VERSION     V011865
KEYWORDS    pAd PL-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 34864)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 34864)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..34864
                     /mol_type="genomic DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..103
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     misc_signal     191..341
                     /label="Ad5 Psi"
                     /note="packaging signal for adenovirus serotype 5"
     protein_bind    512..636
                     /label="attR1"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        661..691
                     /label="lac UV5 promoter"
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             745..1401
                     /label="CmR"
                     /note="chloramphenicol acetyltransferase"
     CDS             1746..2048
                     /label="ccdB"
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(2092..2216)
                     /label="attR2"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2330..2749
                     /gene="IX"
                     /label="Hexon-interlacing protein"
                     /note="Hexon-interlacing protein from Human adenovirus C
                     serotype 5. Accession#: P03281"
     CDS             complement(2815..4149)
                     /gene="IVa2"
                     /label="Packaging protein 1"
                     /note="Packaging protein 1 from Human adenovirus C serotype
                     5. Accession#: P03271"
     CDS             complement(3921..7502)
                     /gene="POL"
                     /label="DNA polymerase"
                     /note="DNA polymerase from Human adenovirus C serotype 2.
                     Accession#: P03261"
     CDS             complement(7307..9310)
                     /gene="PTP"
                     /label="Preterminal protein"
                     /note="Preterminal protein from Human adenovirus C serotype
                     5. Accession#: P04499"
     CDS             9771..11015
                     /gene="L1"
                     /label="Packaging protein 3"
                     /note="Packaging protein 3 from Human adenovirus C serotype
                     5. Accession#: P04496"
     CDS             11039..12793
                     /note="Pre-hexon-linking protein IIIa from Human adenovirus
                     C serotype 5. Accession#: P12537"
                     /label="Pre-hexon-linking protein IIIa"
     CDS             12877..14589
                     /gene="L2"
                     /label="Penton protein"
                     /note="Penton protein from Human adenovirus C serotype 5.
                     Accession#: P12538"
     CDS             14599..15192
                     /gene="L2"
                     /label="Pre-histone-like nucleoprotein"
                     /note="Pre-histone-like nucleoprotein from Human adenovirus
                     C serotype 2. Accession#: P68950"
     CDS             15265..16368
                     /gene="L2"
                     /label="Core-capsid bridging protein"
                     /note="Core-capsid bridging protein from Human adenovirus C
                     serotype 5. Accession#: P24938"
     CDS             16399..16638
                     /note="Pre-core protein X from Human adenovirus C serotype
                     5. Accession#: Q2KS10"
                     /label="Pre-core protein X"
     CDS             16724..17473
                     /gene="L3"
                     /label="Pre-protein VI"
                     /note="Pre-protein VI from Human adenovirus C serotype 5.
                     Accession#: P24937"
     CDS             17563..20418
                     /gene="L3"
                     /label="Hexon protein"
                     /note="Hexon protein from Human adenovirus C serotype 5.
                     Accession#: P04133"
     CDS             20454..21065
                     /gene="L3"
                     /label="Protease"
                     /note="Protease from Human adenovirus C serotype 5.
                     Accession#: P03253"
     CDS             complement(21167..22753)
                     /gene="DBP"
                     /label="DNA-binding protein"
                     /note="DNA-binding protein from Human adenovirus C serotype
                     5. Accession#: P03265"
     CDS             22782..25202
                     /gene="L4"
                     /label="Shutoff protein"
                     /note="Shutoff protein from Human adenovirus C serotype 5.
                     Accession#: P24933"
     CDS             25895..26575
                     /gene="L4"
                     /label="Pre-hexon-linking protein VIII"
                     /note="Pre-hexon-linking protein VIII from Human adenovirus
                     C serotype 5. Accession#: P24936"
     CDS             27885..29627
                     /note="Fiber protein from Human adenovirus C serotype 5.
                     Accession#: P11818"
                     /label="Fiber protein"
     CDS             complement(30039..30920)
                     /note="Early 4 ORF6 protein from Human adenovirus C
                     serotype 2. Accession#: P03239"
                     /label="Early 4 ORF6 protein"
     CDS             complement(31199..31546)
                     /note="Early 4 ORF3 protein from Human adenovirus C
                     serotype 2. Accession#: P03241"
                     /label="Early 4 ORF3 protein"
     CDS             complement(31987..32370)
                     /note="Early 4 ORF1 protein from Human adenovirus C
                     serotype 2. Accession#: P03242"
                     /label="Early 4 ORF1 protein"
     repeat_region   32680..32782
                     /label="ITR"
                     /note="inverted terminal repeat of human adenovirus
                     serotype 5"
     rep_origin      complement(33006..33594)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(33749..34606)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(34607..34711)
                     /label="AmpR promoter"