pEXP1-DEST vector (V011793)

LOCUS       pEXP1-DEST.        4622 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Gateway(R) destination vector for cell-free expression and 
            N-terminal tagging.
ACCESSION   .
VERSION     .
KEYWORDS    pEXP1-DEST
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             100..102
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             112..129
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             133..165
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
     CDS             169..192
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
     protein_bind    202..326
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        351..381
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             435..1091
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     CDS             1436..1738
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
     protein_bind    complement(1782..1906)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      1983..2030
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2127..2582
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2663..2767
                     /label=AmpR promoter
     CDS             2768..3625
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3799..4387
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"