pME18S-FL3 vector (V011718)

MSDS

Basic Vector Information

Vector Name:: pME18S-FL3

Antibiotic Resistance:: Ampicillin

Length:: 3392 bp

Type:: I.M.A.G.E. Consortium Plasmids

Replication origin:: ori

Copy Number:: High copy number

Promoter:: SRα

pME18S-FL3 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pME18S-FL3 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pME18S-FL3.        3392 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for directional cloning and transient expression of
            a cDNA.
ACCESSION   .
VERSION     .
KEYWORDS    pME18S-FL3.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3392)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     A cDNA inserted between the DraIII sites can be excised with XhoI.
FEATURES             Location/Qualifiers
     source          1..3392
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        18..632
                     /label=SR-alpha promoter
                     /note="hybrid promoter consisting of the SV40 early
                     promoter plus part of the long terminal repeat of human 
                     T-lymphotrophic virus 1"
     intron          662..755
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    812..1194
                     /label=stuffer
                     /note="stuffer"
     polyA_signal    1297..1431
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1641..2229)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2403..3260)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3261..3365)
                     /label=AmpR promoter

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