pAB-6xHis-MBP vector (V011703)

LOCUS       V011703                10833 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011703
VERSION     V011703
KEYWORDS    pAB-6xHis-MBP
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 10833)
  AUTHORS   AB Vector
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 10833)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10833
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             76..579
                     /gene="PTP"
                     /label="Tyrosine-protein phosphatase"
                     /note="Tyrosine-protein phosphatase from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P24656"
     CDS             2662..3291
                     /gene="LEF-2"
                     /label="Primase-associated factor LEF-2"
                     /note="Primase-associated factor LEF-2 from Autographa
                     californica nuclear polyhedrosis virus. Accession#: P41418"
     misc_recomb     3170..3997
                     /label="baculovirus recombination region (lef2/ORF603)"
                     /note="contains ORF603 and part of lef2"
     promoter        4001..4092
                     /label="polyhedrin promoter"
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4138..4140
                     /codon_start=1
                     /product="start codon"
                     /label="start codon"
                     /note="ATG"
                     /translation="M"
     CDS             4147..4164
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             4192..5283
                     /label="MBP"
                     /note="maltose binding protein from E. coli"
     CDS             5302..5319
                     /label="thrombin site"
                     /note="thrombin recognition and cleavage site"
     misc_recomb     5848..7303
                     /label="baculovirus recombination region (ORF1629)"
                     /note="contains part of ORF1629"
     promoter        complement(8262..8292)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8307..8328)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(8616..9204)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(9378..10235)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(10236..10340)
                     /label="AmpR promoter"
     primer_bind     10814..10830
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"