Basic Vector Information
- Vector Name:
- pAc5.1/V5-His B
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5379 bp
- Type:
- Insect Cell Vectors
- Replication origin:
- ori
- Source/Author:
- Invitrogen (Life Technologies)
- Copy Number:
- High copy number
- Promoter:
- Ac5
pAc5.1/V5-His B vector Map
pAc5.1/V5-His B vector Sequence
LOCUS pAc5.1_V5-His_B. 5379 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for constitutive expression of C-terminally V5-6xHis-tagged
proteins in insect cells. For other reading frames, use
pAc5.1/V5-His A or pAc5.1/V5-His C.
ACCESSION .
VERSION .
KEYWORDS pAc5.1 V5-His B
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5379)
AUTHORS Invitrogen (Life Technologies)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5379)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT To create stable cell lines, co-transfect with the drug selection
vector pCoHygro or pCoBlast.
FEATURES Location/Qualifiers
source 1..5379
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 56..2568
/label=Ac5 promoter
/note="Drosophila melanogaster actin 5C promoter"
misc_feature 2579..2663
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS 2664..2705
/label=V5 tag
/note="epitope tag from simian virus 5"
CDS 2715..2732
/label=6xHis
/note="6xHis affinity tag"
polyA_signal complement(2805..2939)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3561..4149)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4323..5180)
/label=AmpR
/note="beta-lactamase"
promoter complement(5181..5285)
/label=AmpR promoter
This page is informational only.