pACEBac2 vector (V011690)

LOCUS       pACEBac2.        2761 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Baculovirus transfer vector with a p10 promoter and a gentamycin 
            resistance marker. Serves as an acceptor vector in the MultiBac(TM) 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    pACEBac2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2761)
  AUTHORS   Geneva Biotech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2761)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2761
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      190..778
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(913..1136)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1206..1736)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(1925..1953)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(2024..2057)
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    complement(2273..2321)
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     misc_feature    2386..2449
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(2457..2566)
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     mobile_element  complement(2596..2761)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"