Basic Vector Information
- Vector Name:
- pIDK
- Antibiotic Resistance:
- Kanamycin
- Length:
- 2281 bp
- Type:
- Insect Cell Vectors
- Replication origin:
- R6K γ ori
- Source/Author:
- Geneva Biotech
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
- Promoter:
- p10
pIDK vector Map
pIDK vector Sequence
LOCUS pIDK. 2281 bp DNA circular SYN 01-JAN-1980
DEFINITION Baculovirus transfer vector with a p10 promoter and a kanamycin
resistance marker. Serves as a donor vector in the MultiBac(TM)
system.
ACCESSION .
VERSION .
KEYWORDS pIDK
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2281)
AUTHORS Geneva Biotech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 2281)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..2281
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 15..124
/label=p10 promoter
/note="baculovirus promoter for expression in insect cells"
misc_feature 132..195
/label=MCS
/note="MCS"
/note="multiple cloning site"
polyA_signal 260..308
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
protein_bind 510..543
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
rep_origin 545..901
/label=R6K gamma ori
/note="gamma replication origin from E. coli plasmid R6K;
requires the R6K initiator protein pi for replication"
CDS 1262..2053
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
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