Basic Vector Information
- Vector Name:
- pTriEx-2 Hygro
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7107 bp
- Type:
- Insect Cell Vectors
- Replication origin:
- ori
- Source/Author:
- Novagen (EMD Millipore)
- Copy Number:
- High copy number
- Promoter:
- chicken β-actin
pTriEx-2 Hygro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTriEx-2 Hygro vector Sequence
LOCUS pTriEx-2_Hygro. 7107 bp DNA circular SYN 01-JAN-1980 DEFINITION Insect, bacterial, and mammalian vector with a hygromycin resistance marker, encoding an N-terminal 6xHis-S-Tag cassette and a C-terminal HSV-8xHis cassette. ACCESSION . VERSION . KEYWORDS pTriEx-2 Hygro. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7107) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 7107) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..7107 /mol_type="other DNA" /organism="synthetic DNA construct" misc_recomb 181..1008 /label=baculovirus recombination region (lef2/ORF603) /note="contains ORF603 and part of lef2" enhancer 1140..1443 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 1449..1726 /label=chicken beta-actin promoter exon 1727..1819 /note="Exon 1" intron 1820..2081 promoter 2150..2168 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" protein_bind 2169..2193 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter 2209..2318 /label=p10 promoter /note="baculovirus promoter for expression in insect cells" RBS 2320..2325 /note="ribosome binding site" CDS 2333..2335 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 2339..2356 /label=6xHis /note="6xHis affinity tag" CDS 2366..2410 /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" CDS 2426..2443 /label=thrombin site /note="thrombin recognition and cleavage site" CDS 2462..2476 /label=enterokinase site /note="enterokinase recognition and cleavage site" CDS 2600..2632 /label=HSV tag /note="HSV (herpes simplex virus) epitope tag" CDS 2639..2662 /label=8xHis /note="8xHis affinity tag" misc_feature 2716..3179 /label=IRES /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" CDS 3218..4234 /label=HygR /note="aminoglycoside phosphotransferase from E. coli" polyA_signal 4467..4522 /label=beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" terminator 4610..4657 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" misc_recomb 4670..5375 /label=baculovirus recombination region (ORF1629) /note="contains part of ORF1629" protein_bind complement(5384..5417) /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." rep_origin complement(5585..6172) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(6188..7045) /label=AmpR /note="beta-lactamase"
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