Basic Vector Information
pTriEx-3 Hygro vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTriEx-3 Hygro vector Sequence
LOCUS pTriEx-3_Hygro. 6732 bp DNA circular SYN 01-JAN-1980
DEFINITION Insect, bacterial, and mammalian vector with a CMV promoter and a
hygromycin resistance marker, for expressing proteins with a
C-terminal HSV-8xHis cassette.
ACCESSION .
VERSION .
KEYWORDS pTriEx-3 Hygro.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6732)
AUTHORS Novagen (EMD Millipore)
TITLE Direct Submission
REFERENCE 2 (bases 1 to 6732)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..6732
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_recomb 181..1008
/label=baculovirus recombination region (lef2/ORF603)
/note="contains ORF603 and part of lef2"
enhancer 1076..1379
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1381..1580
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
exon 1597..1698
/note="Exon 1"
intron 1699..1862
promoter 1931..1949
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1950..1974
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 1990..2099
/label=p10 promoter
/note="baculovirus promoter for expression in insect cells"
RBS 2101..2106
/note="ribosome binding site"
misc_feature 2112..2219
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS 2114..2116
/codon_start=1
/product="start codon"
/label=start codon
/note="ATG"
/translation="M"
CDS 2225..2257
/label=HSV tag
/note="HSV (herpes simplex virus) epitope tag"
CDS 2264..2287
/label=8xHis
/note="8xHis affinity tag"
misc_feature 2341..2804
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 2843..3859
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
polyA_signal 4092..4147
/label=beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal (Gil and
Proudfoot, 1987)"
terminator 4235..4282
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
misc_recomb 4295..5000
/label=baculovirus recombination region (ORF1629)
/note="contains part of ORF1629"
protein_bind complement(5009..5042)
/label=loxP
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (ATGTATGC) (Shaw et al., 2021)."
rep_origin complement(5210..5797)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5813..6670)
/label=AmpR
/note="beta-lactamase"
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