Basic Vector Information
- Vector Name:
- pVL-FH
- Antibiotic Resistance:
- Ampicillin
- Length:
- 9723 bp
- Type:
- Insect Cell Vectors
- Replication origin:
- ori
- Source/Author:
- AB Vector
- Copy Number:
- High copy number
- Promoter:
- polyhedrin
pVL-FH vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVL-FH vector Sequence
LOCUS pVL-FH. 9723 bp DNA circular SYN 01-JAN-1980
DEFINITION Baculovirus transfer plasmid for high-level expression of proteins
with a C-terminal FLAG(R)-8xHis cassette.
ACCESSION .
VERSION .
KEYWORDS pVL-FH.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9723)
AUTHORS AB Vector
TITLE Direct Submission
REFERENCE 2 (bases 1 to 9723)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9723
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 76..579
/gene="PTP"
/label=PTP
/note="Tyrosine-protein phosphatase from Autographa
californica nuclear polyhedrosis virus. Accession#: P24656"
CDS 2662..3291
/gene="LEF-2"
/label=LEF-2
/note="Primase-associated factor LEF-2 from Autographa
californica nuclear polyhedrosis virus. Accession#: P41418"
misc_recomb 3170..3997
/label=baculovirus recombination region (lef2/ORF603)
/note="contains ORF603 and part of lef2"
promoter 4001..4092
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
CDS 4176..4199
/label=FLAG
/note="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
CDS 4209..4232
/label=8xHis
/note="8xHis affinity tag"
misc_recomb 4738..6193
/label=baculovirus recombination region (ORF1629)
/note="contains part of ORF1629"
primer_bind complement(7104..7120)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7128..7144)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7152..7182)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7197..7218)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7506..8094)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8268..9125)
/label=AmpR
/note="beta-lactamase"
promoter complement(9126..9230)
/label=AmpR promoter
primer_bind 9704..9720
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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