Basic Vector Information
pVL1393 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pVL1393 vector Sequence
LOCUS pVL1393. 9639 bp DNA circular SYN 01-JAN-1980
DEFINITION Baculovirus transfer vector containing the polyhedrin promoter, for
use with the BD BaculoGold(TM) system. For an alternative MCS, see
pVL1392.
ACCESSION .
VERSION .
KEYWORDS pVL1393.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9639)
AUTHORS BD Biosciences
TITLE Direct Submission
REFERENCE 2 (bases 1 to 9639)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..9639
/mol_type="other DNA"
/organism="synthetic DNA construct"
CDS 76..579
/gene="PTP"
/label=PTP
/note="Tyrosine-protein phosphatase from Autographa
californica nuclear polyhedrosis virus. Accession#: P24656"
CDS 2662..3291
/gene="LEF-2"
/label=LEF-2
/note="Primase-associated factor LEF-2 from Autographa
californica nuclear polyhedrosis virus. Accession#: P41418"
misc_recomb 3170..3997
/label=baculovirus recombination region (lef2/ORF603)
/note="contains ORF603 and part of lef2"
promoter 4001..4092
/label=polyhedrin promoter
/note="promoter for the baculovirus polyhedrin gene"
misc_feature 4093..4095
/label=mutated start codon
/note="mutated start codon"
/note="the ATG start codon of polyhedrin was mutated to
ATT"
misc_feature 4129..4192
/label=MCS
/note="MCS"
/note="multiple cloning site"
misc_recomb 4654..6109
/label=baculovirus recombination region (ORF1629)
/note="contains part of ORF1629"
primer_bind complement(7020..7036)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(7044..7060)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7068..7098)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(7113..7134)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(7422..8010)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(8184..9041)
/label=AmpR
/note="beta-lactamase"
promoter complement(9042..9146)
/label=AmpR promoter
primer_bind 9620..9636
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
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