pFN26A (BIND) hRluc-neo Flexi Vector vector (V011550)

LOCUS       pFN26A_(BIND)_hR        7070 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Flexi(R) Vector encoding Renilla luciferase fused to a neomycin 
            resistance gene. This vector is useful for testing transcriptional 
            activation domains.
ACCESSION   .
VERSION     .
KEYWORDS    pFN26A (BIND) hRluc-neo Flexi Vector
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7070)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The protein-coding region of interest can be subcloned between the 
            SgfI (AsiSI) and PmeI sites.
FEATURES             Location/Qualifiers
     source          1..7070
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1083..1523
                     /label=GAL4 DNA binding domain
                     /note="DNA binding domain of the GAL4 transcriptional
                     activator"
     CDS             1527..1553
                     /codon_start=1
                     /product="peptide derived from a human Ig alpha chain"
                     /label=peptide derived from a human Ig alpha chain
                     /note="IgA linker"
                     /translation="PSTPPTPSP"
     CDS             1585..1917
                     /label=barnase
                     /note="ribonuclease from Bacillus amyloliquefaciens"
     misc_feature    1930..1985
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     polyA_signal    complement(2090..2211)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2456..2813
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2864..3796
                     /label=hRluc
                     /note="Renilla luciferase"
     CDS             3848..4636
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4703..4751
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        4907..5011
                     /label=AmpR promoter
     CDS             5012..5869
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(6030..6618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    6734..7017
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"