pGL4.15[luc2P/Hygro] vector (V011535)

LOCUS       pGL4.15[luc2P_Hy        5969 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Promoterless vector encoding destabilized luciferase for measuring 
            the activity of promoter and enhancer sequences.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.15[luc2P Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             100..1749
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             1753..1872
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(1924..2045)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2239..2596
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2627..3661
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    3688..3736
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4063..4651)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4854..5711)
                     /label=AmpR
                     /note="beta-lactamase"
     polyA_signal    5816..5864
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     misc_feature    5878..5969
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"