pGL4.37[luc2P/ARE/Hygro] vector (V011513)

LOCUS       pGL4.37[luc2P_AR        6086 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a minimal promoter and an antioxidant response element 
            for studying cell signaling using destabilized luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pGL4.37[luc2P ARE Hygro]
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6086)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6086)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6086
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    105..153
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    167..258
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
     protein_bind    285..366
                     /label=NF-E2 p45-related factor 2 (Nrf2) binding site
                     /bound_moiety="NF-E2 p45-related factor 2 (Nrf2)"
                     /note="ARE"
                     /note="two copies of the antioxidant response element from
                     the mouse glutathione S-transferase Ya gene"
     promoter        411..442
                     /label=minP
                     /note="minimal TATA-box promoter with low basal activity"
     CDS             475..2124
                     /label=luciferase
                     /note="firefly luciferase"
     CDS             2128..2247
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
     polyA_signal    complement(2299..2420)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2614..2971
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             3002..4036
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
     polyA_signal    4063..4111
                     /label=synthetic polyadenylation signal
                     /note="synthetic polyadenylation signal"
     rep_origin      complement(4438..5026)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5229..6086)
                     /label=AmpR
                     /note="beta-lactamase"