Basic Vector Information
- Vector Name:
- pGL4.80[hRlucP/Neo]
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5010 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Promega
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
pGL4.80[hRlucP/Neo] vector Map
pGL4.80[hRlucP/Neo] vector Sequence
LOCUS pGL4.80[hRlucP_N 5010 bp DNA circular SYN 01-JAN-1980
DEFINITION Promoterless vector encoding destabilized Renilla luciferase for
measuring the activity of promoter and enhancer sequences.
ACCESSION .
VERSION .
KEYWORDS pGL4.80[hRlucP Neo]
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5010)
AUTHORS Promega
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5010)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..5010
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 1..70
/label=MCS
/note="MCS"
/note="multiple cloning site"
CDS 100..1032
/label=hRluc
/note="Renilla luciferase"
CDS 1036..1155
/label=hPEST
/note="PEST degradation sequence from mouse ornithine
decarboxylase"
polyA_signal complement(1207..1328)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter 1522..1879
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 1910..2701
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 2729..2777
/label=poly(A) signal
/note="synthetic polyadenylation signal"
rep_origin complement(3104..3692)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3895..4752)
/label=AmpR
/note="beta-lactamase"
polyA_signal 4857..4905
/label=synthetic polyadenylation signal
/note="synthetic polyadenylation signal"
misc_feature 4919..5010
/label=pause site
/note="RNA polymerase II transcriptional pause signal from
the human alpha-2 globin gene"
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