phRL-SV40 vector (V011477)

Basic Vector Information

      • Vector Name:
      • phRL-SV40
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3705 bp
      • Type:
      • Luciferase Vectors
      • Source/Author:
      • Promega
      • Copy Number:
      • High copy number

phRL-SV40 vector Vector Map

phRL-SV403705 bp60012001800240030003600SV40 promoterchimeric intronT7 promoterhRlucSV40 poly(A) signalAmpR promoterAmpRori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

phRL-SV40 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       40924_24977        3705 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector phRL-SV40, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Zhuang Y, Kenefick KB, Kopps P.
  TITLE     phRL-SV40 Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Zhuang Y, Kenefick KB, Kopps P.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 3705)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods 
            Hollow Rd., Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..1626
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(1660..1781)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1914..2018
                     /label=AmpR promoter
     CDS             2019..2876
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      3050..3638
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"

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