pLVX-MetLuc Control vector (V011473)

MSDS

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V011473	pLVX-MetLuc Control	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

pLVX-MetLuc Control is a lentiviral vector for constitutive expression of secreted Metridia luciferase, as a control. It can applied with another vector pLVX-MetLuc Reporter vector (V011472).

Vector Name:: pLVX-MetLuc Control

Antibiotic Resistance:: Ampicillin

Length:: 8721 bp

Type:: Luciferase Vectors

Replication origin:: ori

Source/Author:: Clontech

Copy Number:: High copy number

Promoter:: mPGK

pLVX-MetLuc Control vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

Liu X, Chen JG, Munshi M, Hunter ZR, Xu L, Kofides A, Tsakmaklis N, Demos MG, Guerrera ML, Chan GG, Jimenez C, Patterson CJ, Meid K, Keezer A, Castillo JJ, Treon SP, Yang G. Expression of the prosurvival kinase HCK requires PAX5 and mutated MYD88 signaling in MYD88-driven B-cell lymphomas. Blood Adv. 2020 Jan 14;4(1):141-153.

pLVX-MetLuc Control vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pLVX-MetLuc_Cont        8721 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Lentiviral vector for constitutive expression of secreted Metridia 
            luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-MetLuc Control.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8721)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8721)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8721
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2027..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2201..2504
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2505..2708
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    2802..2867
                     /label=MCS
                     /note="multiple cloning site"
     CDS             2880..3536
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
     promoter        3556..4055
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4076..4672
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    4689..5277
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5484..6117
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6246..6262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(6270..6286)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6294..6324)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(6339..6360)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6648..7236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7410..8267)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(8268..8372)
                     /label=AmpR promoter
     polyA_signal    8420..8554
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"