pLVX-MetLuc Reporter vector (V011472)

MSDS

Price Information

Cat No.	Plasmid Name	Availability	Add to cart
V011472	pLVX-MetLuc Reporter	In stock (lyophilized plasmid)	Buy one, get one free!

Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.

Basic Vector Information

The pLVX-MetLuc Reporter is a lentiviral vector for measuring the activity of promoter and enhancer sequences using secreted Metridia luciferase. The selection marker is puromycin.

Vector Name:: pLVX-MetLuc Reporter

Antibiotic Resistance:: Ampicillin

Length:: 8088 bp

Type:: Luciferase Vectors

Replication origin:: ori

Source/Author:: Clontech

Copy Number:: High copy number

Promoter:: mPGK

pLVX-MetLuc Reporter vector Vector Map

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Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

References

Liu X, Chen JG, Munshi M, Hunter ZR, Xu L, Kofides A, Tsakmaklis N, Demos MG, Guerrera ML, Chan GG, Jimenez C, Patterson CJ, Meid K, Keezer A, Castillo JJ, Treon SP, Yang G. Expression of the prosurvival kinase HCK requires PAX5 and mutated MYD88 signaling in MYD88-driven B-cell lymphomas. Blood Adv. 2020 Jan 14;4(1):141-153.

pLVX-MetLuc Reporter vector Sequence

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Download GeneBank File(.gb)

LOCUS       pLVX-MetLuc_Repo        8088 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Lentiviral vector for measuring the activity of promoter and 
            enhancer sequences using secreted Metridia luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pLVX-MetLuc Reporter.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2027..2144
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     misc_feature    2182..2234
                     /label=MCS
                     /note="multiple cloning site"
     CDS             2247..2903
                     /label=MetLuc
                     /note="secreted Metridia luciferase"
     promoter        2923..3422
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             3443..4039
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     misc_feature    4056..4644
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             4851..5484
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(5613..5629)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5637..5653)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5661..5691)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5706..5727)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(6015..6603)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6777..7634)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7635..7739)
                     /label=AmpR promoter
     polyA_signal    7787..7921
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"