Basic Vector Information
- Vector Name:
- pMCS-Green Renilla Luc
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5263 bp
- Type:
- Luciferase Vectors
- Replication origin:
- ori
- Source/Author:
- Thermo Scientific (Pierce)
- Copy Number:
- High copy number
- Promoter:
- EM7
pMCS-Green Renilla Luc vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pMCS-Green Renilla Luc vector Sequence
LOCUS pMCS-Green_Renil 5263 bp DNA circular SYN 01-JAN-1980 DEFINITION Intracellular green Renilla luciferase vector for cloning a transcriptional regulatory element. ACCESSION . VERSION . KEYWORDS pMCS-Green Renilla Luc. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5263) AUTHORS Thermo Scientific (Pierce) TITLE Direct Submission REFERENCE 2 (bases 1 to 5263) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..5263 /mol_type="other DNA" /organism="synthetic DNA construct" misc_feature 1..42 /label=MCS /note="MCS" /note="multiple cloning site" CDS 49..981 /label=Green Renilla Luciferase /note="green-emitting variant of Renilla luciferase" polyA_signal 1007..1118 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" promoter 1241..1570 /label=SV40 promoter /note="SV40 enhancer and early promoter" promoter 1618..1665 /label=EM7 promoter /note="synthetic bacterial promoter" CDS 1684..2280 /label=PuroR /note="puromycin N-acetyltransferase" polyA_signal 2413..2546 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(2590..3447) /label=AmpR /note="beta-lactamase" rep_origin 3711..4299 /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4569..4585 /label=M13 fwd /note="common sequencing primer, one of multiple similar variants" protein_bind 5041..5057 /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." misc_feature 5172..5263 /label=pause site /note="RNA polymerase II transcriptional pause signal from the human alpha-2 globin gene"
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