pMCS-Green Renilla Luc vector (V011468)

LOCUS       pMCS-Green_Renil        5263 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Intracellular green Renilla luciferase vector for cloning a 
            transcriptional regulatory element.
ACCESSION   .
VERSION     .
KEYWORDS    pMCS-Green Renilla Luc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5263)
  AUTHORS   Thermo Scientific (Pierce)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5263)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..42
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             49..981
                     /label=Green Renilla Luciferase
                     /note="green-emitting variant of Renilla luciferase"
     polyA_signal    1007..1118
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        1241..1570
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        1618..1665
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter"
     CDS             1684..2280
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     polyA_signal    2413..2546
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(2590..3447)
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      3711..4299
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4569..4585
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5041..5057
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     misc_feature    5172..5263
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"