pSF-CMV-PGK-FLuc vector (V011437)

LOCUS       pSF-CMV-PGK-FLuc        6394 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Dual-promoter mammalian vector for strong constitutive expression of
            a gene together with firefly luciferase.
ACCESSION   .
VERSION     .
KEYWORDS    pSF-CMV-PGK-FLuc
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Oxford Genetics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The PGK promoter is typically about 10-fold weaker than the CMV 
            promoter.
FEATURES             Location/Qualifiers
     source          1..6394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    18..89
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     terminator      complement(94..141)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    complement(158..206)
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     enhancer        287..590
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        591..794
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    857..942
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     RBS             893..898
                     /label=Shinbe-Dalgarno ribosome binding site
                     /note="Shinbe-Dalgarno ribosome binding site"
     misc_signal     903..909
                     /label=Kozak sequence
                     /note="Kozak sequence"
     CDS             907..909
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     misc_feature    961..971
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        988..1487
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             1498..3147
                     /label=luciferase
                     /note="firefly luciferase"
     misc_feature    3158..3168
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     polyA_signal    complement(3200..3321)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     terminator      3435..3482
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      3512..3648
                     /note="rrnG terminator"
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     misc_feature    3693..3764
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus
                     (Farrell et al., 2002)"
     rep_origin      complement(3990..4578)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     RBS             5311..5316
                     /label=Shine-Dalgarno ribosome binding site
                     /note="Shine-Dalgarno ribosome binding site"
     CDS             5324..6115
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     terminator      6131..6267
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal
                     RNA rrnG operon (Albrechtsen et al., 1991)"