pAviTag-N N-His SUMO Kan vector (V011403)

LOCUS       pAviTag-N_N-His        2659 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for Expresso(R) cloning to add N-terminal 6xHis, 
            biotinylation, and SUMO tags, with rhamnose-inducible expression.
ACCESSION   .
VERSION     .
KEYWORDS    pAviTag-N N-His SUMO Kan
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Lucigen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..119
                     /label=rhaB promoter
                     /note="promoter of the E. coli rhaBAD operon, conferring
                     tight induction with L-rhamnose and repression with 
                     D-glucose in the presence of RhaR and RhaS (Giacalone et 
                     al., 2006)"
     RBS             153..175
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             183..185
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             186..203
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             204..248
                     /label=AviTag(TM)
                     /note="peptide tag that allows for enzymatic biotinylation"
     CDS             300..587
                     /label=SUMO
                     /note="cleavable ubiquitin-like protein tag"
     terminator      619..666
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      764..793
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     rep_origin      815..1402
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      complement(1414..1441)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             complement(1468..1656)
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     CDS             complement(1660..2472)
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(2473..2563)
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     terminator      complement(2564..2595)
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription
                     terminator"