p3xFLAG-CMV-7 vector (V011359)

Basic Vector Information

      • Vector Name:
      • p3xFLAG-CMV-7
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 4690 bp
      • Type:
      • Mammalian Expression Vectors
      • Source/Author:
      • Sigma-Aldrich
      • Copy Number:
      • High copy number
      • 5' Primer:
      • M13 fwd
      • 3' Primer:
      • M13 rev

p3xFLAG-CMV-7 vector Vector Map

p3xFLAG-CMV-74690 bp600120018002400300036004200M13 fwdCMV enhancerCMV promoterATG3xFLAGhGH poly(A) signalSV40 promoterT7 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterf1 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p3xFLAG-CMV-7 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       p3xFLAG-CMV-7.        4690 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression of N-terminally 3xFLAG-tagged proteins in 
            mammalian cells. For a longer MCS, use p3xFLAG-CMV(TM)-7.1.
ACCESSION   .
VERSION     .
KEYWORDS    p3xFLAG-CMV-7.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Sigma-Aldrich
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4690
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             928..930
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             931..996
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     misc_feature    994..1033
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1031..1653
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1682..2011
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2050..2068)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2082..2098)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2106..2122)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2130..2160)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2175..2196)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2484..3072)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3246..4103)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4104..4208)
                     /label=AmpR promoter
     rep_origin      4235..4690
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"

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