p3xFLAG-CMV-8 vector (V011358)

LOCUS       p3xFLAG-CMV-8.        4763 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expression and secretion of N-terminally 3xFLAG-tagged 
            proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    p3xFLAG-CMV-8
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4763)
  AUTHORS   Sigma-Aldrich
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4763)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4763
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             932..976
                     /label=preprotrypsin leader
                     /note="leader sequence from mouse preprotrypsin"
     CDS             977..1042
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     misc_feature    1040..1106
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1104..1726
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1755..2084
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        complement(2123..2141)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2155..2171)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2179..2195)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2203..2233)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2248..2269)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2557..3145)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3319..4176)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4177..4281)
                     /label=AmpR promoter
     rep_origin      4308..4763
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"