p3xFLAG-CMV-9 vector (V011357)

Basic Vector Information

      • Vector Name:
      • p3xFLAG-CMV-9
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 6373 bp
      • Type:
      • Mammalian Expression Vectors
      • Source/Author:
      • Sigma-Aldrich
      • Selection Marker:
      • Neomycin/G418(Geneticin)
      • Copy Number:
      • High copy number
      • 5' Primer:
      • M13 fwd
      • 3' Primer:
      • M13 rev

p3xFLAG-CMV-9 vector Vector Map

p3xFLAG-CMV-96373 bp300600900120015001800210024002700300033003600390042004500480051005400570060006300M13 fwdCMV enhancerCMV promoterpreprotrypsin leader3xFLAGhGH poly(A) signalSV40 promoterNeoR/KanRSV40 poly(A) signalT7 promoterM13 revlac operatorlac promoterCAP binding siteoriAmpRAmpR promoterf1 ori

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

p3xFLAG-CMV-9 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       p3xFLAG-CMV-9.        6373 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a neomycin resistance marker for expression and 
            secretion of N-terminally 3xFLAG-tagged proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    p3xFLAG-CMV-9.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6373)
  AUTHORS   Sigma-Aldrich
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6373)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             942..986
                     /label=preprotrypsin leader
                     /note="leader sequence from mouse preprotrypsin"
     CDS             987..1052
                     /label=3xFLAG
                     /note="three tandem FLAG(R) epitope tags, followed by an 
                     enterokinase cleavage site"
     misc_feature    1050..1116
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1114..1736
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1778..2076
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2130..2921
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3576..3710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3716..3734)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3748..3764)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3772..3788)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3796..3826)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3841..3862)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4150..4738)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4912..5769)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5770..5874)
                     /label=AmpR promoter
     rep_origin      5901..6373
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"

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