pALTER-MAX vector (V011352)

LOCUS       pALTER-MAX.        5538 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector with the CMV enhancer/promoter and with 
            ampicillin and chloramphenicol resistance markers.
ACCESSION   .
VERSION     .
KEYWORDS    pALTER-MAX
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5538)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5538)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The AmpR gene was edited to correct an apparent error in the GenBank
            sequence.
FEATURES             Location/Qualifiers
     source          1..5538
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..729
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          890..1022
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1067..1085
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    1091..1137
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        complement(1145..1162)
                     /note="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    complement(1180..1301)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      1487..1942
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2014..2371
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     promoter        2520..2622
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding
                     chloramphenicol acetyltransferase"
     CDS             2623..3279
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     polyA_signal    3379..3427
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        3741..3845
                     /label=AmpR promoter
     CDS             3846..4703
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      4877..5465
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"