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pBI-CMV3 vector (V011349)

Basic Vector Information

Vector Name:
pBI-CMV3
Antibiotic Resistance:
Ampicillin
Length:
3798 bp
Type:
Mammalian Expression Vectors
Replication origin:
ori
Source/Author:
Clontech
Copy Number:
High copy number

pBI-CMV3 vector Map

Copy Sequence View Map
pBI-CMV33798 bp60012001800240030003600CMV enhancerCMV promoterMCSSV40 poly(A) signaloriAmpRAmpR promoterSV40 poly(A) signalZsGreen1minimal CMV promoter

pBI-CMV3 vector Sequence

LOCUS       pBI-CMV3.        3798 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bidirectional promoter vector for expressing a gene together with 
            the fluorescent protein ZsGreen1.
ACCESSION   .
VERSION     .
KEYWORDS    pBI-CMV3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     The gene inserted into the MCS should contain start and stop codons.
FEATURES             Location/Qualifiers
     source          1..3798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        67..370
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        371..574
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    602..663
                     /label=MCS 1
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    777..858
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1040..1628)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1802..2659)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2660..2764)
                     /label=AmpR promoter
     polyA_signal    complement(2797..2878)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(3020..3712)
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     promoter        complement(3753..3790)
                     /note="minimal CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"

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