pCMV-DDK-C vector (V011253)

LOCUS       pCMV-DDK-C.        3766 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for CMV promoter-driven expression of an ORF with a
            C-terminal FLAG(R) tag.
ACCESSION   .
VERSION     .
KEYWORDS    pCMV-DDK-C
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3766)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3766)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3766
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        27..330
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        331..534
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          672..768
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     misc_feature    832..863
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     CDS             867..890
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     polyA_signal    complement(905..1039)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1150..1166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1174..1190)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1198..1228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1243..1264)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(1552..2140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2314..3171)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3172..3276)
                     /label=AmpR promoter
     primer_bind     3750..3766
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"