pFLAG-CMV-3 vector (V011138)

LOCUS       pFLAG-CMV-3.        6331 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector with a neomycin resistance marker for expression and 
            secretion of N-terminally FLAG-tagged proteins in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    pFLAG-CMV-3
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6331)
  AUTHORS   Sigma-Aldrich
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6331)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     This sequence provided by Sigma-Aldrich may contain errors in the 
            SV40 promoter and f1 ori regions. See pFLAG-CMV(TM)-4 for 
            comparison.
FEATURES             Location/Qualifiers
     source          1..6331
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     141..157
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        318..697
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        698..901
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             942..986
                     /label=preprotrypsin leader
                     /note="leader sequence from mouse preprotrypsin"
     CDS             987..1010
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
     misc_feature    1008..1074
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    1072..1694
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     promoter        1736..2034
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2088..2879
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3534..3668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(3674..3692)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3706..3722)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3730..3746)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3754..3784)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3799..3820)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4108..4696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4870..5727)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5728..5832)
                     /label=AmpR promoter
     rep_origin      5859..6331
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"