phCMV2 vector (V011119)

LOCUS       phCMV2.        4261 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Compact mammalian cell vector for high-level expression of 
            N-terminally HA-tagged proteins.
ACCESSION   .
VERSION     .
KEYWORDS    phCMV2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4261)
  AUTHORS   MoBiTec
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4261)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          704..799
                     /label=CMV intron
                     /note="modified intron A from human cytomegalovirus (CMV)"
     CDS             817..819
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             820..846
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     misc_feature    855..911
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1049..1170
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1177..1632)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1659..1763
                     /label=AmpR promoter
     promoter        1765..2122
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2157..2948
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3183..3230
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      3559..4147
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"