pHTN HaloTag CMV-neo vector (V011110)

LOCUS       pHTN_HaloTag_CMV        6143 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian expression vector with a traditional MCS and ampicillin 
            and G418 resistance markers, encoding a cleavable N-terminal 
            HaloTag(R).
ACCESSION   .
VERSION     .
KEYWORDS    pHTN HaloTag CMV-neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6143)
  AUTHORS   Promega
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6143)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6143
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        139..517
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        518..721
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          857..989
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        1033..1051
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1067..1957
                     /label=HaloTag(R)
                     /note="modified bacterial dehalogenase that forms covalent
                     bonds with chloroalkane derivatives"
     CDS             1970..1990
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     misc_feature    1997..2072
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    complement(2144..2265)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        2510..2867
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2918..3709
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3776..3824
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        3980..4084
                     /label=AmpR promoter
     CDS             4085..4942
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      complement(5103..5691)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    5807..6090
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to
                     maintain plasmids as monomers"