Basic Vector Information
- Vector Name:
- pNTAP-A
- Antibiotic Resistance:
- Kanamycin
- Length:
- 4530 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- Agilent Technologies
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
pNTAP-A vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNTAP-A vector Sequence
LOCUS pNTAP-A. 4530 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for expressing proteins N-terminally tagged with calmodulin-
and streptavidin-binding peptides. For other reading frames, use
pNTAP-B or pNTAP-C.
ACCESSION .
VERSION .
KEYWORDS pNTAP-A.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4530)
AUTHORS Agilent Technologies
TITLE Direct Submission
REFERENCE 2 (bases 1 to 4530)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..4530
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 67..370
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 371..574
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 620..638
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
CDS 679..681
/codon_start=1
/product="start codon"
/label=start codon
/note="ATG"
/translation="M"
CDS 682..759
/label=CBP
/note="calmodulin-binding peptide"
CDS 778..891
/label=SBP
/note="streptavidin-binding peptide"
primer_bind complement(959..975)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 994..1004
/label=stop codons
/note="stop codons"
/note="stop codons in all three reading frames"
promoter complement(1032..1050)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
polyA_signal 1324..1445
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(1452..1907)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1934..2036
/label=AmpR promoter
promoter 2040..2397
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 2432..3223
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase"
polyA_signal 3458..3505
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 3834..4422
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
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