Basic Vector Information
- Vector Name:
- pOG44
- Antibiotic Resistance:
- Ampicillin
- Length:
- 5785 bp
- Type:
- Mammalian Expression Vectors
- Replication origin:
- ori
- Source/Author:
- Ben Glick
- Copy Number:
- High copy number
- Promoter:
- CMVd2
pOG44 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pOG44 vector Sequence
LOCUS 40924_33727 5785 bp DNA circular SYN 01-JAN-1980
DEFINITION Vector for constitutive expression of a thermolabile Flp recombinase
(flp-F70L) in mammalian cells, for use with the Flp-In(TM) system.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5785)
AUTHORS Ben Glick
TITLE Direct Submission
REFERENCE 2 (bases 1 to 5785)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
COMMENT This plasmid does not contain a drug resistance marker, and will be
gradually lost during cell culture.
FEATURES Location/Qualifiers
source 1..5785
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 237..616
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 617..820
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 865..883
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
intron 932..1161
/label=chimeric intron
/note="chimera between introns from adenovirus and
immunoglobulin heavy chain genes"
CDS 1202..2470
/codon_start=1
/label=FLP
/note="site-specific recombinase"
/translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
THNGTAIKRATFMSYNTIISNSLSLDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
YPAWNGIISQEVLDYLSSYINRRI"
polyA_signal complement(2610..2731)
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(2945..2963)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(2984..3000)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind complement(3008..3024)
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3032..3062)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(3077..3098)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(3386..3967)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4141..4998)
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4999..5103)
/label=AmpR promoter
rep_origin complement(5128..5583)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind 5725..5741
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
This page is informational only.