pSingle-tTS-shRNA vector (V011080)

LOCUS       V011080                 6919 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V011080
VERSION     V011080
KEYWORDS    pSingle-tTs-shRNA
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6919)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6919)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            Clone an annealed double-stranded oligonucleotide encoding the shRNA
            into the vector digested with XhoI and HindIII.
FEATURES             Location/Qualifiers
     source          1..6919
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..246
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     CDS             289..1080
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    1315..1363
                     /label="HSV TK poly(A) signal"
                     /note="HSV TK poly(A) signal"
                     /note="herpesvirus thymidine kinase polyadenylation signal"
     enhancer        1449..1828
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1829..2032
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             2135..2980
                     /label="tTS"
                     /note="fusion of a hybrid tetracycline repressor TetR with
                     a nuclear localization signal and a KRAB silencing domain"
     intron          3004..3576
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     CDS             3580..3702
                     /gene="HBB"
                     /label="Hemoglobin subunit beta"
                     /note="Hemoglobin subunit beta from Colobus guereza.
                     Accession#: Q9TT33"
     polyA_signal    3773..3828
                     /label="beta-globin poly(A) signal"
                     /note="rabbit beta-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     promoter        4246..4577
                     /label="TREmod/U6 promoter"
                     /note="modified Tet-responsive promoter, consisting of
                     seven repeats of the tet operator fused to a portion of the
                     human U6 promoter"
     rep_origin      complement(5100..5688)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5862..6719)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6720..6824)
                     /label="AmpR promoter"