Basic Vector Information
pTandem-1 vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pTandem-1 vector Sequence
LOCUS pTandem-1. 3813 bp DNA circular SYN 01-JAN-1980 DEFINITION Mammalian vector for the co-expression of two genes from a bicistronic mRNA using the CMV promoter and an IRES. ACCESSION . VERSION . KEYWORDS pTandem-1. SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3813) AUTHORS Novagen (EMD Millipore) TITLE Direct Submission REFERENCE 2 (bases 1 to 3813) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article" FEATURES Location/Qualifiers source 1..3813 /mol_type="other DNA" /organism="synthetic DNA construct" enhancer 56..359 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 361..560 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" intron 679..842 promoter 911..929 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" RBS 961..983 /label=RBS /note="efficient ribosome binding site from bacteriophage T7 gene 10 (Olins and Rangwala, 1989)" CDS 993..995 /codon_start=1 /product="start codon" /label=start codon /note="ATG" /translation="M" CDS 999..1016 /label=6xHis /note="6xHis affinity tag" CDS 1026..1070 /label=S-Tag /note="affinity and epitope tag derived from pancreatic ribonuclease A" CDS 1086..1103 /label=thrombin site /note="thrombin recognition and cleavage site" CDS 1122..1136 /label=enterokinase site /note="enterokinase recognition and cleavage site" CDS 1233..1265 /label=HSV tag /note="HSV (herpes simplex virus) epitope tag" CDS 1272..1295 /label=8xHis /note="8xHis affinity tag" misc_feature 1321..1805 /label=IRES /note="IRES" /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" misc_feature 1806..1848 /label=MCS 2 /note="MCS 2" /note="multiple cloning site 2" polyA_signal 1913..1968 /label=beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" terminator 2056..2103 /label=T7 terminator /note="transcription terminator for bacteriophage T7 RNA polymerase" rep_origin complement(2275..2862) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(2878..3735) /label=AmpR /note="beta-lactamase" promoter complement(3736..3806) /label=AmpR promoter promoter 3812..3813 /label=CMV /note="Human cytomegalovirus immediate early enhancer/promoter"
This page is informational only.