pTandem-1 vector (V011077)

Basic Vector Information

      • Vector Name:
      • pTandem-1
      • Antibiotic Resistance:
      • Ampicillin
      • Length:
      • 3813 bp
      • Type:
      • Mammalian Expression Vectors
      • Source/Author:
      • Novagen (EMD Millipore)
      • Copy Number:
      • High copy number

pTandem-1 vector Vector Map

pTandem-13813 bp60012001800240030003600CMV enhancerCMV promoterT7 promoterRBSATG6xHisS-Tagthrombin siteenterokinase siteHSV tag8xHisIRESMCS 2beta-globin poly(A) signalT7 terminatororiAmpRAmpR promoterCMV

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTandem-1 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pTandem-1.        3813 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for the co-expression of two genes from a 
            bicistronic mRNA using the CMV promoter and an IRES.
ACCESSION   .
VERSION     .
KEYWORDS    pTandem-1.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        56..359
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        361..560
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          679..842
     promoter        911..929
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             961..983
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             993..995
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             999..1016
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1026..1070
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1086..1103
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1122..1136
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             1233..1265
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     CDS             1272..1295
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    1321..1805
                     /label=IRES
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1806..1848
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     polyA_signal    1913..1968
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     terminator      2056..2103
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(2275..2862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2878..3735)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3736..3806)
                     /label=AmpR promoter
     promoter        3812..3813
                     /label=CMV
                     /note="Human cytomegalovirus immediate early
                     enhancer/promoter"

This page is informational only.