pTandem-1 vector (V011077)

LOCUS       pTandem-1.        3813 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector for the co-expression of two genes from a 
            bicistronic mRNA using the CMV promoter and an IRES.
ACCESSION   .
VERSION     .
KEYWORDS    pTandem-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3813)
  AUTHORS   Novagen (EMD Millipore)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3813)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        56..359
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        361..560
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          679..842
     promoter        911..929
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             961..983
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             993..995
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             999..1016
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             1026..1070
                     /label=S-Tag
                     /note="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
     CDS             1086..1103
                     /label=thrombin site
                     /note="thrombin recognition and cleavage site"
     CDS             1122..1136
                     /label=enterokinase site
                     /note="enterokinase recognition and cleavage site"
     CDS             1233..1265
                     /label=HSV tag
                     /note="HSV (herpes simplex virus) epitope tag"
     CDS             1272..1295
                     /label=8xHis
                     /note="8xHis affinity tag"
     misc_feature    1321..1805
                     /label=IRES
                     /note="IRES"
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    1806..1848
                     /label=MCS 2
                     /note="MCS 2"
                     /note="multiple cloning site 2"
     polyA_signal    1913..1968
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     terminator      2056..2103
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(2275..2862)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2878..3735)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(3736..3806)
                     /label=AmpR promoter
     promoter        3812..3813
                     /label=CMV
                     /note="Human cytomegalovirus immediate early
                     enhancer/promoter"