pTet-Off vector (V011071)

MSDS

Basic Vector Information

Vector Name:: pTet-Off

Antibiotic Resistance:: Ampicillin

Length:: 7400 bp

Type:: Mammalian Expression Vectors

Replication origin:: ori

Source/Author:: Resnitzky D, Gossen M, Bujard H, Reed SI.

Selection Marker:: Neomycin/G418(Geneticin)

Copy Number:: High copy number

Promoter:: SV40

pTet-Off vector Vector Map

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pTet-Off vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pTet-Off.        7400 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing the tTA transactivator in the Tet-Off(R) 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    pTet-Off.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Resnitzky D, Gossen M, Bujard H, Reed SI.
  TITLE     Acceleration of the G1/S phase transition by expression of cyclins 
            D1 and E with an inducible system.
  JOURNAL   Mol. Cell. Biol. 1994;14:1669-79.
  PUBMED    8114703
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7400)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Cell. 
            Biol."; date: "1994"; volume: "14"; pages: "1669-79"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Also known as pUHD15-1neo.
FEATURES             Location/Qualifiers
     source          1..7400
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        89..468
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        469..672
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             774..1778
                     /label=tTA
                     /note="tetracycline-controlled transactivator, comprising a
                     fusion of the tetracycline repressor TetR with the 
                     C-terminal activation domain of herpes simplex virus VP16"
     polyA_signal    1792..1926
                     /label=SV40 poly(A) signal
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2646..3234)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3408..4265)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4266..4370)
                     /label=AmpR promoter
     polyA_signal    complement(4483..4617)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(5042..5062)
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     intron          complement(5192..5257)
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(5680..6471)
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     promoter        complement(6832..7161)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"

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