pTRE-Tight vector (V011056)

LOCUS       pTRE-Tight.        2605 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing a gene with the Tet-On(R) Advanced or 
            Tet-Off(R) Advanced system.
ACCESSION   .
VERSION     .
KEYWORDS    pTRE-Tight
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..2605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        4..318
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     misc_feature    323..411
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     polyA_signal    519..600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(782..1370)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1544..2401)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(2402..2506)
                     /label=AmpR promoter