pGEX-3X vector (V010919)

LOCUS       pGEX-3X.        4952 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Bacterial vector for expressing GST fusions with a Factor Xa site.
ACCESSION   .
VERSION     .
KEYWORDS    pGEX-3X
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   GE Healthcare
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4952
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        183..211
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    219..235
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             258..911
                     /label=GST
                     /note="glutathione S-transferase from Schistosoma
                     japonicum"
     CDS             921..932
                     /label=Factor Xa site
                     /note="Factor Xa recognition and cleavage site"
     misc_feature    934..949
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    954..964
                     /label=stop codons
                     /note="stop codons"
                     /note="stop codons in all three reading frames"
     promoter        1255..1359
                     /label=AmpR promoter
     CDS             1360..2217
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2391..2979
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        3223..3300
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             3301..4380
                     /label=lacI
                     /note="lac repressor"
     protein_bind    4396..4417
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4432..4462
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     CDS             4506..4679
                     /label=lacZ-alpha
                     /note="LacZ-alpha fragment of beta-galactosidase"