pCAMBIA3201 vector (V010870)

LOCUS       pCAMBIA3201.       11472 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Agrobacterium binary vector for plant transformation, with 
            bialophos/phosphinothricin resistance and chloramphenicol resistance
            and GUS genes.
ACCESSION   .
VERSION     .
KEYWORDS    pCAMBIA3201
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11472)
  AUTHORS   Cambia
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 11472)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..11472
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          17..206
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             201..2024
                     /label=GUS
                     /note="beta-glucuronidase"
     CDS             2031..2048
                     /label=6xHis
                     /note="6xHis affinity tag"
     terminator      2083..2335
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    2357..2381
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             3681..4307
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
     CDS             4739..5809
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     rep_origin      5878..6072
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     misc_feature    6416..6556
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(6742..7330)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(7604..8260)
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     misc_feature    8788..8812
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     polyA_signal    complement(8890..9064)
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(9074..9622)
                     /label=BlpR
                     /note="phosphinothricin acetyltransferase"
     promoter        complement(9667..10344)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     protein_bind    10535..10556
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        10571..10601
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10609..10625
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     10633..10649
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    10659..10715
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(10719..10735)
                     /label=M13 fwd
                     /note="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        11112..11457
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"