pQE-T7-1 vector (V010770)

LOCUS       pQE-T7-1.        5317 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing N-terminally 10xHis-tagged proteins in 
            bacteria using the QIAgenes system.
ACCESSION   .
VERSION     .
KEYWORDS    pQE-T7-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5317)
  AUTHORS   Qiagen
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5317)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     A QIAgene can be cloned into this vector as an NdeI-XhoI fragment.
FEATURES             Location/Qualifiers
     source          1..5317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     CDS             complement(140..157)
                     /label=6xHis
                     /note="6xHis affinity tag"
     misc_feature    158..217
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     misc_feature    166..171
                     /label=stop codons
                     /note="stop codons"
                     /note="two tandem stop codons"
     CDS             complement(218..244)
                     /label=9xHis
                     /note="9xHis affinity tag"
     CDS             complement(248..250)
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     RBS             complement(258..280)
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(295..319)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(320..338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        647..724
                     /label=lacI promoter
     CDS             725..1804
                     /label=lacI
                     /note="lac repressor"
     protein_bind    1820..1841
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             2616..2804
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2909..3048
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3234..3822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             3944..4756
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     rep_origin      complement(4851..5306)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"