pMCSG40 vector (V010661)

MSDS

Basic Vector Information

Vector Name:: pMCSG40

Antibiotic Resistance:: Ampicillin

Length:: 6796 bp

Type:: Structural Genomics Vectors

Replication origin:: ori

Source/Author:: Midwest Center for Structural Genomics

Copy Number:: High copy number

Promoter:: dsbA

pMCSG40 vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMCSG40 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V010661                 6796 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010661
VERSION     V010661
KEYWORDS    pMCSG40.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 6796)
  AUTHORS   Midwest Center for Structural Genomics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 6796)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            For ligation-independent cloning (LIC), linearize with SspI and
            treat with T4 DNA polymerase plus dGTP.
FEATURES             Location/Qualifiers
     source          1..6796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(140..157)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(223..243)
                     /label="TEV site"
                     /note="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
     CDS             complement(268..285)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(286..306)
                     /label="TVMV site"
                     /note="tobacco vein mottling virus (TVMV) NIa protease
                     recognition and cleavage site"
     CDS             complement(328..930)
                     /gene="osmY"
                     /label="Osmotically-inducible protein Y"
                     /note="Osmotically-inducible protein Y from Escherichia
                     coli (strain K12). Accession#: P0AFH8"
     RBS             complement(938..960)
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(975..999)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1000..1018)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        1236..1467
                     /label="dsbA promoter"
                     /note="promoter for the E. coli dsbA gene (Belin and
                     Boquet, 1994)"
     CDS             1468..2091
                     /label="DsbA"
                     /note="bacterial periplasmic oxidoreductase"
     promoter        2199..2276
                     /label="lacI promoter"
     CDS             2277..3356
                     /label="lacI"
                     /note="lac repressor"
     protein_bind    3372..3393
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             4168..4356
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    4461..4603
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4789..5377)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(5551..6408)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(6409..6512)
                     /label="AmpR promoter"

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