pMCSG79 vector (V010593)

LOCUS       pMCSG79.        4815 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Transposon-containing vector with a 6xHis-TEV leader, for production
            of recombinant proteins with the Bac-to-Bac(R) baculovirus system.
ACCESSION   .
VERSION     .
KEYWORDS    pMCSG79
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4815)
  AUTHORS   Midwest Center for Structural Genomics
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 4815)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     For ligation-independent cloning (LIC), linearize with XcmI and 
            treat with T4 DNA polymerase plus dGTP.
FEATURES             Location/Qualifiers
     source          1..4815
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      2..457
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        484..588
                     /label=AmpR promoter
     CDS             589..1446
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1620..2208
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  complement(2511..2735)
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(2805..3335)
                     /label=GmR
                     /note="gentamycin acetyltransferase"
     promoter        complement(3524..3552)
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        3904..3995
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             4050..4052
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             4053..4070
                     /label=6xHis
                     /note="6xHis affinity tag"
     CDS             4095..4115
                     /label=TEV site
                     /note="tobacco etch virus (TEV) protease recognition and 
                     cleavage site"
     polyA_signal    4305..4439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  complement(4468..4633)
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"