Basic Vector Information
- Vector Name:
- pNH-TrxT
- Antibiotic Resistance:
- Kanamycin
- Length:
- 7602 bp
- Type:
- Structural Genomics Vectors
- Replication origin:
- ori
- Source/Author:
- Savitsky P, Bray J, Cooper CD, Marsden BD, Mahajan P, Burgess-Brown
- Copy Number:
- High copy number
- Promoter:
- sacB
pNH-TrxT vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pNH-TrxT vector Sequence
LOCUS 40924_33187 7602 bp DNA circular SYN 18-DEC-2018
DEFINITION Expression vector pNH-TrxT, complete sequence.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7602)
AUTHORS Cooper CDO., Gileadi O.
TITLE
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 7602)
AUTHORS Cooper CDO., Gileadi O.
TITLE Direct Submission
JOURNAL Submitted (04-DEC-2009) Structural Genomics Consortium, University
of Oxford, ORCRB, Old Road Campus, off Roosevelt Drive, Oxford,
Oxfordshire OX3 7DQ, United Kingdom
REFERENCE 3 (bases 1 to 7602)
TITLE Direct Submission
REFERENCE 4 (bases 1 to 7602)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
(04-DEC-2009) Structural Genomics Consortium, University of Oxford,
ORCRB, Old Road Campus, off Roosevelt Drive, Oxford, Oxfordshire OX3
7DQ, United Kingdom"
COMMENT SGRef: number: 3; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7602
/mol_type="other DNA"
/organism="synthetic DNA construct"
RBS 12..34
/label=RBS
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 45..62
/label=6xHis
/note="6xHis affinity tag"
CDS 72..398
/label=TrxA
/note="E. coli thioredoxin"
CDS 405..425
/label=TEV site
/note="tobacco etch virus (TEV) protease recognition and
cleavage site"
promoter 441..886
/label=sacB promoter
/note="sacB promoter and control region"
CDS 887..2305
/label=SacB
/note="secreted levansucrase that renders bacterial growth
sensitive to sucrose"
misc_feature 2354..2368
/note="3'-LIC sequence; add complement to downstream PCR
primer"
CDS 2416..2433
/label=6xHis
/note="6xHis affinity tag"
terminator 2500..2547
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 2584..3039
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
CDS complement(3135..3947)
/label=KanR
/note="aminoglycoside phosphotransferase"
rep_origin 4069..4657
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature complement(4843..4985)
/label=bom
/note="basis of mobility region from pBR322"
CDS complement(5090..5278)
/label=rop
/note="Rop protein, which maintains plasmids at low copy
number"
protein_bind complement(6053..6074)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
CDS complement(6090..7169)
/label=lacI
/note="lac repressor"
promoter complement(7170..7247)
/label=lacI promoter
promoter 7556..7574
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 7575..7599
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
This page is informational only.