pBABE-Puro vector (V010490)

MSDS

Basic Vector Information

Vector Name:: pBABE-Puro

Antibiotic Resistance:: Ampicillin

Length:: 5169 bp

Type:: Viral Expression & Packaging Vectors

Replication origin:: ori

Source/Author:: Morgenstern JP, Land H.

Selection Marker:: Puromycin

Copy Number:: High copy number

Cloning Method:: Restriction Enzyme

5' Primer:: pBABE 5'

3' Primer:: pBABE 3'

pBABE-Puro vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pBABE-Puro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pBABE-Puro.        5169 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral expression vector derived from the Moloney murine 
            leukemia virus. Contains a puromycin resistance marker.
ACCESSION   .
VERSION     .
KEYWORDS    pBABE-Puro.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5169)
  AUTHORS   Morgenstern JP, Land H.
  TITLE     Advanced mammalian gene transfer: high titre retroviral vectors with
            multiple drug selection markers and a complementary helper-free 
            packaging cell line.
  JOURNAL   Nucleic Acids Res. 1990;18:3587-96.
  PUBMED    2194165
REFERENCE   2  (bases 1 to 5169)
  AUTHORS   Cell Biolabs
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 5169)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "1990"; volume: "18"; pages: "3587-96"
COMMENT     SGRef: number: 2; type: "Journal Article"
COMMENT     Strong expression is driven by the viral promoter in the 5' LTR. The
            commercially provided sequence was modified to include the splice 
            donor mutation.
FEATURES             Location/Qualifiers
     source          1..5169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..477
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     misc_feature    541
                     /label=splice donor mutation
                     /note="splice donor mutation"
                     /note="T-to-C mutation that blocks RNA splicing"
     misc_feature    542..741
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus
                     (MMLV)"
     CDS             934..1350
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     misc_feature    1355..1402
                     /label=MCS
                     /note="MCS"
                     /note="multiple cloning site"
     promoter        1413..1742
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             1752..2348
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             2450..3043
                     /label=LTR
                     /note="long terminal repeat from Moloney murine leukemia
                     virus"
     rep_origin      complement(3415..4003)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4184..5041)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(5042..5146)
                     /label=AmpR promoter

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