pLenti6/TR vector (V010461)

LOCUS       V010461                 8354 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V010461
VERSION     V010461
KEYWORDS    pLenti6 TR
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Invitrogen (Life Technologies)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8354)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8354
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1075..1308
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1493..1537
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1686..1727
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     enhancer        1816..2119
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2120..2323
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     intron          2552..3124
                     /label="beta-globin intron"
                     /note="intron from rabbit beta-globin gene"
     promoter        3179..3197
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             3208..3828
                     /label="TetR"
                     /note="tetracycline repressor TetR"
     CDS             3870..3899
                     /label="Myc"
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
     promoter        3947..4276
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        4324..4371
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             4390..4785
                     /label="BSD"
                     /note="blasticidin S deaminase"
     LTR             4875..5108
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5180..5314
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      5341..5476
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(5497..5515)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5525..5541)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      5683..6138
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6164..6268
                     /label="AmpR promoter"
     CDS             6269..7126
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7300..7888
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     protein_bind    8176..8197
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8212..8242
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8250..8266
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8274..8290
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        8311..8329
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"