pLKO.1 puro vector (V010450)

Price Information

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V010450 pLKO.1 puro In stock, instant shipping

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Two tubes of lyophilized plasmid will be delivered, each tube is about 5µg.

Basic Vector Information

pLKO.1 puro is a 3rd gen lentiviral backbone for cloning and expression of new shRNA sequences. The selection marker is puromycin.

Vector Name:
pLKO.1 puro
Antibiotic Resistance:
7050 bp
Viral Expression & Packaging Vectors
Replication origin:
Weinberg Lab / Addgene
Selection Marker:
Copy Number:
High copy number
Growth Strain(s):
Growth Temperature:

pLKO.1 puro vector Vector Map

pLKO.1 puro7050 bp30060090012001500180021002400270030003300360039004200450048005100540057006000630066006900hPGK promoterPuroR3' LTR (Delta-U3)SV40 poly(A) signalSV40 oriT7 promoterM13 fwdf1 oriAmpR promoterAmpRoriCAP binding sitelac promoterlac operatorM13 revT3 promoterRSV promoter5' LTR (truncated)HIV-1 PsiRREgp41 peptideProtein TatU6 promotercPPT/CTShPGK promoter

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.


  • Kanungo J. Puromycin-resistant lentiviral control shRNA vector, pLKO.1 induces unexpected cellular differentiation of P19 embryonic stem cells. Biochem Biophys Res Commun. 2017 Apr 29;486(2):481-485.
  • Takenaka C, Nishishita N, Takada N, Jakt LM, Kawamata S. Effective generation of iPS cells from CD34+ cord blood cells by inhibition of p53. Exp Hematol. 2010 Feb;38(2):154-62. doi: 10.1016/j.exphem.2009.11.003. Epub 2009 Nov 14. PMID: 19922768.

pLKO.1 puro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       V010450                 7050 bp    DNA     circular SYN 01-JAN-1980
VERSION     V010450
KEYWORDS    pLKO.1 puro
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7050)
  AUTHORS   Weinberg Lab / Addgene
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7050)
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
            The sequence from the depositing scientist was corrected using
            information from Addgene's plasmid 8453.
FEATURES             Location/Qualifiers
     source          1..7050
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        7..507
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             529..1125
                     /note="puromycin N-acetyltransferase"
     LTR             1256..1489
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
     polyA_signal    1561..1695
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      1722..1857
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(1878..1896)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1906..1922)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
     rep_origin      2064..2519
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2545..2649
                     /label="AmpR promoter"
     CDS             2650..3507
     rep_origin      3681..4269
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
     protein_bind    4557..4578
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        4593..4623
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4631..4647
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4655..4671
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
     promoter        4692..4710
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4738..4964
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4965..5145
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5192..5317
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    5810..6043
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
     CDS             6228..6272
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             6421..6462
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        6570..6810
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    6881..6998
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        7047..7050
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"