pLMN-ZsGreen-Neomycin vector (V010448)

LOCUS       pLMN-ZsGreen-Neo        8065 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Retroviral vector with a ZsGreen marker, for expressing a short 
            hairpin RNA (shRNA) with an """"UltramiR"""" microRNA scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    pLMN-ZsGreen-Neomycin
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   transOMIC
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..515
                     /label=MSCV
                     /note="Murine embryonic stem cell virus promoter including
                     the enhancer and promoter region of PCMV virus and 5' 
                     untranslated region of dl-587rev retrovirus"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     ncRNA           1419..1540
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the
                     human miR-30a microRNA (Zeng et al., 2002)"
     misc_RNA        1541..1609
                     /label=shRNA
                     /note="shRNA"
                     /note="short hairpin RNA"
     gap             1544..1565
                     /estimated_length=22
     gap             1585..1606
                     /estimated_length=22
     ncRNA           1610..1736
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the
                     human miR-30a microRNA (Zeng et al., 2002)"
     promoter        1757..2256
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             2270..3070
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     misc_feature    3083..3658
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             3659..4351
                     /label=ZsGreen1
                     /note="Zoanthus green fluorescent protein"
     LTR             4458..4972
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem
                     cell virus"
     protein_bind    complement(5134..5150)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5158..5188)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5203..5224)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5512..6100)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6274..7131)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(7132..7236)
                     /label=AmpR promoter
     LTR             8064..8065
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"