Price Information
| Cat No. | Plasmid Name | Availability | Add to cart |
|---|---|---|---|
| V010422 | pLVX-Tet-Off Advanced | In stock (lyophilized plasmid) |
Buy one, get one free! |
Two vials of lyophilized plasmid will be delivered, each vial is about 5µg.
Basic Vector Information
- Vector Name:
- pLVX-Tet-Off Advanced
- Antibiotic Resistance:
- Ampicillin
- Length:
- 11625 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- Clontech
- Selection Marker:
- Neomycin/G418(Geneticin)
- Copy Number:
- High copy number
pLVX-Tet-Off Advanced vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLVX-Tet-Off Advanced vector Sequence
LOCUS pLVX-Tet-Off_Adv 11625 bp DNA circular SYN 01-JAN-1980
DEFINITION Lentiviral vector for producing a doxycycline-inhibited
transactivator in a Tet-Off system.
ACCESSION .
VERSION .
KEYWORDS pLVX-Tet-Off Advanced.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11625)
AUTHORS Clontech
TITLE Direct Submission
REFERENCE 2 (bases 1 to 11625)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..11625
/mol_type="other DNA"
/organism="synthetic DNA construct"
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
CDS 1914..1955
/note="Protein Tat from Human immunodeficiency virus type 1
group M subtype B (isolate WMJ22). Accession#: P12509"
misc_feature 2027..2144
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2201..2504
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2505..2708
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2797..3540
/label=tTA-Advanced
/note="improved tetracycline-controlled transactivator"
misc_feature 3570..4156
/label=IRES2
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 4157..4948
/label=NeoR/KanR
/note="aminoglycoside phosphotransferase from Tn5"
misc_feature 5147..5735
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 5942..6575
/label=5' LTR
/note="5' long terminal repeat (LTR) from HIV-1"
polyA_signal 6688..6912
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6958..7386
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 7400..7729
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
CDS 7778..8800
/label=HygR
/note="aminoglycoside phosphotransferase from E. coli"
polyA_signal 8933..9066
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
promoter complement(9151..9181)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind complement(9196..9217)
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(9505..10093)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(10267..11124)
/label=AmpR
/note="beta-lactamase"
promoter complement(11125..11229)
/label=AmpR promoter
polyA_signal 11277..11411
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"