Basic Vector Information
- Vector Name:
- pLX304-V5-Blast-Empty
- Antibiotic Resistance:
- Ampicillin
- Length:
- 7671 bp
- Type:
- Viral Expression & Packaging Vectors
- Replication origin:
- ori
- Source/Author:
- transOMIC
- Copy Number:
- High copy number
- Promoter:
- hPGK
pLX304-V5-Blast-Empty vector Vector Map
Plasmid Resuspension Protocol:
1. Centrifuge at 5,000×g for 5 min.
2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.
5.Store the plasmid at -20 ℃.
pLX304-V5-Blast-Empty vector Sequence
LOCUS 40924_29476 7671 bp DNA circular SYN 01-JAN-1980
DEFINITION Empty control vector for the ORF collection in the pLX304 lentiviral
vector.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7671)
AUTHORS transOMIC
TITLE Direct Submission
REFERENCE 2 (bases 1 to 7671)
AUTHORS .
TITLE Direct Submission
COMMENT SGRef: number: 1; type: "Journal Article"
FEATURES Location/Qualifiers
source 1..7671
/mol_type="other DNA"
/organism="synthetic DNA construct"
enhancer 65..368
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 369..572
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 595..636
/codon_start=1
/label=V5 tag
/note="epitope tag from simian virus 5"
/translation="GKPIPNPLLGLDST"
misc_feature 678..1266
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 1338..1571
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 1649..1783
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 1810..1945
/label=SV40 ori
/note="SV40 origin of replication"
promoter complement(1966..1984)
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(1994..2010)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
rep_origin 2152..2607
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2633..2737
/label=AmpR promoter
CDS 2738..3595
/codon_start=1
/label=AmpR
/note="beta-lactamase"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3769..4357
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 4645..4666
/label=CAP binding site
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 4681..4711
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4719..4735
/label=lac operator
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 4743..4759
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
promoter 4780..4798
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
promoter 4826..5052
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 5053..5233
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 5280..5405
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 5898..6131
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 6316..6360
/codon_start=1
/label=gp41 peptide
/note="antigenic peptide corresponding to amino acids 655
to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
al., 2013)"
/translation="KNEQELLELDKWASL"
promoter 6535..7035
/label=hPGK promoter
/note="human phosphoglycerate kinase 1 promoter"
CDS 7047..7442
/codon_start=1
/label=BSD
/note="blasticidin S deaminase"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 7502..7619
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
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