pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro vector (V010403)

MSDS

Basic Vector Information

Vector Name:: pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro

Antibiotic Resistance:: Ampicillin

Length:: 7602 bp

Type:: Viral Expression & Packaging Vectors

Replication origin:: ori

Source/Author:: Cellecta

Copy Number:: High copy number

Promoter:: MSCV

pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro vector Vector Map

View Map in NovoBuilder

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       pMSCV16URP-U6-sh        7602 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Lentiviral vector derived from murine embryonic stem cell virus, for
            barcoded shRNA libraries, with TagRFP and puromycin resistance 
            markers.
ACCESSION   .
VERSION     .
KEYWORDS    pMSCV16URP-U6-sh-HTS6-UbiC-TagRFP-2A-Puro.
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   Cellecta
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 7602)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..515
                     /label=MSCV
                     /note="Murine embryonic stem cell virus promoter including
                     the enhancer and promoter region of PCMV virus and 5' 
                     untranslated region of dl-587rev retrovirus"
     misc_feature    579..920
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell
                     virus"
     CDS             987..1403
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag
                     gene lacking the start codon"
     promoter        1438..1678
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    1684..1713
                     /label=cloning site
                     /note="cloning site"
                     /note="shRNA cloning site"
     promoter        1872..2271
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     CDS             2284..2994
                     /label=TagRFP
                     /note="monomeric derivative of red fluorescent protein from
                     Entacmaea quadricolor (Merzlyak et al., 2007)"
     CDS             3001..3054
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=2A peptide from Thosea asigna virus capsid
                     protein
                     /note="T2A"
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             3061..3657
                     /label=PuroR
                     /note="puromycin N-acetyltransferase"
     LTR             3736..4161
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     promoter        4427..4756
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      complement(5049..5637)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5811..6668)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(6669..6773)
                     /label=AmpR promoter
     LTR             7601..7602
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem
                     cell virus"

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